Functional Foods Business
Only One Material for People's Health
In order to manufacture safe and high quality products based on our cultivation technology cultivated for over 50 years, we manufacture our products in a hygienic environment and management methods in accordance with Good Manufacturing Practice (GMP) for dietary supplements, including a series of manufacturing processes and quality inspections, to ensure the safety of our products. We strive to ensure that our products are safe and effective. We are committed to providing reliable quality for your valuable products.
Rich in nutrients and energy
Extracts from shiitake mycelial culture medium [LEM].
LEM is "Shiitake mycelium culture medium extract," a useful extract obtained from long-term cultivation of shiitake mycelium in a solid medium consisting of bagasse and non-fat rice bran.
LEM is a useful substance produced by the mycelium of shiitake mushrooms (microorganisms), and thus belongs to the same category of fermented foods as natto, cheese, yogurt, miso, soy sauce, sake, lactic acid beverages, and so on.
![Rich in nutrients and energy Extracts from shiitake mycelial culture medium [LEM].](../_src/87314194/image.webp?v=1744857854550)
Extracts from shiitake mushroom mycelial culture medium
LEM is "Shiitake mycelium culture medium extract," which is a useful extract extract of shiitake mycelium cultivated for a long period of time.
The mycelium of the shiitake mushroom grows by decomposing logs, sawdust, etc., and later develops on the surface of the logs.
LEM is a substance obtained by inoculating the shiitake mushroom fungus into a medium of "bagasse" and "rice sugar" after extracting the sugar component from sugarcane, and extracting by natural means all of the metabolites obtained through the growth of the shiitake mushroom fungus using its own enzymes and the components obtained by decomposing the mycelium of the shiitake mushroom with its own enzymes.
LEM is a member of the same fermented food group as natto, cheese, yogurt, miso, soy sauce, sake, lactic acid drinks, etc. Fermentation is the process by which microorganisms and their enzymes produce substances that are beneficial to humans.
The power of LEM and its evidence
LEM contains two types of active components: heteropolysaccharides and soluble lignin. Heteropolysaccharides arise primarily from cellulose and hemicellulose, which make up cell walls. The sugars that make up heteropolysaccharides are mainly pentasaccharides such as arabinose and xylose. In addition, water-soluble lignin is derived from water-insoluble lignin that is made soluble by breaking down the lignin that makes up the cell wall. LEM has a variety of effects, including immunomodulating, antiviral, antioxidant, and cancer cell growth inhibitory effects.
Safety of LEM
The shiitake mycelium used in the production of LEM is the same as that of edible shiitake mushrooms. Therefore, there are no safety issues. In addition, the safety of LEM has been confirmed through various safety tests, including toxicity and mutagenicity tests, conducted by an analytical institution.
| acute toxicity test LD50 mg/kg/day |
rat | male | 16,400 |
| female | 15,600 | ||
| mouse | male | 19,600 | |
| fermale | 17,700 | ||
| subacute toxicity test maximum no-action volumemg/kg/day |
rat | male | 3,840 |
| fermale | 7,980 | ||
| mouse | male | 6,740 | |
| fermale | 9,100 | ||
| genotoxicity test | gene mutation (Ames test) | negative | |
| DNA damege (Rec assay or umu test) | negative | ||
Example of LEM efficacy test (anti-influenza action)
▒ Inhibition of proliferation
After influenza viruses were infected cultured cells, a given concentration of LEM was added, and plaque area was compared by plaque assay. The results showed that LEM inhibited plaque area expansion in a concentration-dependent manner. This suggested that LEM inhibited the growth of influenza viruses.
The power of LEM and its evidence
LEM contains two types of active components: heteropolysaccharides and soluble lignin. Heteropolysaccharides arise primarily from cellulose and hemicellulose, which make up cell walls. The sugars that make up heteropolysaccharides are mainly pentasaccharides such as arabinose and xylose. In addition, water-soluble lignin is derived from water-insoluble lignin that is made soluble by breaking down the lignin that makes up the cell wall. LEM has a variety of effects, including immunomodulating, antiviral, antioxidant, and cancer cell growth inhibitory effects.
Safety of LEM
The shiitake mycelium used in the production of LEM is the same as that of edible shiitake mushrooms. Therefore, there are no safety issues. In addition, the safety of LEM has been confirmed through various safety tests, including toxicity and mutagenicity tests, conducted by an analytical institution.
| acute toxicity test LD50 mg/kg/day |
rat | male | 16,400 |
| female | 15,600 | ||
| mouse | male | 19,600 | |
| fermale | 17,700 | ||
| subacute toxicity test maximum no-action volumemg/kg/day |
rat | male | 3,840 |
| fermale | 7,980 | ||
| mouse | male | 6,740 | |
| fermale | 9,100 | ||
| genotoxicity test | gene mutation (Ames test) | negative | |
| DNA damege (Rec assay or umu test) | negative | ||
Example of LEM efficacy test (anti-influenza action)
▒ Inhibition of proliferation
After influenza viruses were infected cultured cells, a given concentration of LEM was added, and plaque area was compared by plaque assay. The results showed that LEM inhibited plaque area expansion in a concentration-dependent manner. This suggested that LEM inhibited the growth of influenza viruses.
List of LEM Academic Reports examples
- Kohayakawa S. et al. (2018) Anti-allergic effects of a water-soluble lignin rich fraction (JLS-18) prepared from the extract of culture medium of Lentinus edodes mycelia (LEM). New food industry 60. 42-48. (In Japanese)
- Kuroki T. et al. (2018) Inhibition of influenza virus infection by Lentinus edodes mycelia extract through its direct action and immunopotentiating activity. Frontiers in Microbiology 9. 1164.
- Xuan M. et al. (2017) Cerebroprotective effects of a water-soluble extract from the culture medium of Lentinus edodes New food industry 59. 1-14. (In Japanese)
- Horiuchi S. et al. (2016) Neuroprotective effects of a water-soluble extract from the culture medium of Lentinus edodesmycelia (LEM). New food industry 58. 1-10. (In Japanese)
- Xuan M. et al. (2011) Protective effects of a water-soluble extract from culture medium of Lentinus edodes mycelia against neuronal damage after hypoxia-ischemia in mice. The Japanese Society for Complementary and Alternative Medicine 8. 99-107. (In Japanese)
- Yamamoto Y. et al. (1997) Immunopotentiating activity of the water-soluble lignin rich fraction prepared from LEM -The extract of the solid culture medium of Lentinus edodes Bioscience, Biotechnology, and Biochemistry 61. 1909-1912.
- Morinaga H. et al. (1994) An in vivo study of hepatic and splenic interleukin-1β mRNA expression following oral PSK or LEM administration. Japanese Journal of Cancer Research 85. 1298-1303.
- Hibino Y. et al. (1994) Productions of interferon-γ and nitrite are induced in mouse splenic cells by a heteroglycan-protein fraction from culture medium of Lentinus edodes Immunopharmacology 28. 77-85.
- Sarkar S. et al. (1993) Antiviral effect of the extract of culture medium of Lentinus edodes mycelia on the replication of herpes simplex virus type 1. Antiviral Research 20. 293-303.
- Kajihara J. et al. (1993) Characterization of antiviral water-soluble lignin from bagasse degraded by Lentinus edodes. Holzforshung 47. 479-485.
- Hattori T. et al. (1993) Formate and Oxalate Esters in Lignin Obtained from Bagasse Degraded by Lentinus edodes. Journal of the Japan Wood Research Society 39. 1317-1321.
Mice were infected with influenza virus through their nasal cavity and orally administered LEM for 7 days. As a result, the gross lesions with inflammation, hemorrhage and congestion in the lungs of mice orally administered LEM were much smaller than those of mice not administered LEM. This suggested that LEM suppressed lung inflammation.
We can also provide you with samples of each raw material.
We can also provide you with samples of each raw material.